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KMID : 0381120080300020093
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Genes and Genomics
2008 Volume.30 No. 2 p.93 ~ p.99
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Overexpression of Zinc Finger Protein of Capsicum annuum (PIF1) in Tobacco Enhances Cold Tolerance
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Hwang Eul-Won
Park Soo-Chul
Byun Myung-Ok
Choi Mie-Young
Kwon Hawk-Bin
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Abstract
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Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper (Capsicum annuum ) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold
stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances.
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KEYWORD
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zinc finger protein, Capsicum annuum, transgenic tobacco, cold tolerance
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